Figure 4.

DXZ4 CpG methylation patterns in male and female macaques. (a) Schematic map of a single human 3-kb DXZ4 monomer represented by an open arrow. Distance in kilobases is given along the top. Underneath, the colored bars indicate the regions of DXZ4 corresponding to the defined promoter, CTCF and TFIID binding sites [12] and the 621-bp interval of macaque DXZ4 that was assessed for CpG methylation by bisulfite sequencing. (b) CpG methylation profile of the 621-bp region of macaque DXZ4 in male and female rhesus macaque (R. Macaque) and pig-tailed macaque (P-T. Macaque) samples. All 39 cytosine residues corresponding to CpG dinucleotides within the interval are represented by circles. White circles indicate no methylation whereas black circles indicate a methylated C. Each row of circles represents sequence data from an independent clone. Dashes within the profile represent a sequence that diverged from the consensus and did not have a C residue at that site. The given percentage of methylation is based on the number of methylated residues within the full profile.