Proposed evolutionary relationship between tRNAAsp(GUC) in D. kamchatkensis, A. pernix, and T. aggregans. D. kamchatkensis has a typical linear tRNAAsp(GUC) which represents the likely ancestor of the split tRNAAsp(GUC) genes in A. pernix and T. aggregans. The 5' and 3' halves of pre-tRNAAsp(GUC) are located adjacent to each other in A. pernix and T. aggregans. The two halves in A. pernix are transcribed on the forward strand while those in T. aggregans are transcribed on opposite strands. Breaks in synteny were observed between the tRNA halves and upstream of the 5' half in A. pernix.
Chan et al. Genome Biology 2011 12:R38 doi:10.1186/gb-2011-12-4-r38