Table 1

Summary of trans-spliced and permuted tRNAs

5' half of tRNA

3' half of tRNA


Organism

tRNA isotype

Anticodon

5' Start

Size (bp) Pre/Mature

5' Start

Size (bp) Pre/Mature

Ligation site

Intron position


*Aeropyrum pernix

Asp

GUC

372080 (+)

~67/40

372241 (+)

~58/35

37/38

*Thermosphaera aggregans

Asp

GUC

41001 (+)

~53/40

41160 (-)

~53/35

37/38

*Staphylothermus hellenicus

Lys

CUU

1095046 (+)

~48/31

1090266 (+)

~65/43

30/31

*Staphylothermus marinus

Lys

CUU

1279876 (-)

~48/31

1284654 (-)

~65/43

30/31

Nanoarchaeum equitans

Lys

CUU

308963 (+)

~44/32

380370 (-)

~60/43

30/31

Gln

UUG

419223 (-)

~49/33

356559 (+)

~61/40

32/33

Glu

CUC

331758 (-)

~56/40

487064 (-)

~56/35

37/38

Glu

UUC

436673 (-)

~56/40

487064 (-)

~5635

37/38

His

GUG

221794 (+)

~49/37

409246 (+)

~53/36

37/38

iMet

CAU

35249 (+)

~53/39

3254 (+)

~5236

37/38

*Thermofilum pendens

iMet

CAU

539254 (-)

~89/75

539278 (-)

~43/17

59/60

37/38

Tyr

GUA

892743 (+)

~128/117

892725 (+)

~34/17

59/60

37/38

Cyanidioschyzon merolae

Ala

UGC

chr6:137501 (-)

~63/58

chr6:137523 (-)

~32/14

59/60

Arg

CCU

chr10:636110 (+)

~88/85

chr10:636073 (+)

~47/14

59/60

14/15

Lys

UUU

chr16:809263 (-)

~60/57

chr16:809315 (-)

~70/14

59/60


The novel trans-spliced and permuted tRNAs identified in this study are marked by asterisks. The previously identified split tRNAs in N. equitans [4,5] and permuted tRNAs in C. merolae [9] are listed for comparison. The 5' start value represents the coordinates of the 5' start site of the mature tRNA. Pre, pre-tRNA; Mature, mature tRNA.

Chan et al. Genome Biology 2011 12:R38   doi:10.1186/gb-2011-12-4-r38

Open Data