DNaseI patterns correlate with in situ spatio-temporal expression patterns and demonstrate the high sensitivity of the assay. For each panel, DNAse I tag densities for four genes at stages 5, 9, 10, 11 and 14 are shown in green, orange, red, blue, and purple, respectively. On the left of the accessibility plots for each stage are images from the BDGP in situ mRNA expression database of that gene during the relevant stage. (a,b) Decreases in chromatin accessibility near the pxb (a) and CG10479 (b) genes were associated with concomitant changes in spatio-temporal expression of the genes in vivo. (c,d) Increases in chromatin accessibility through development at the CG9747 (c) and CG9331 (d) genes were associated with increases in expression of the gene in vivo. Even though a relatively low percentage of cells are expressing the CG9747 gene at the latest stage in (c), an associated change in chromatin accessibility is still reflected in the chromatin accessibility profile, demonstrating the sensitivity of the DNaseI assay.
Thomas et al. Genome Biology 2011 12:R43 doi:10.1186/gb-2011-12-5-r43