Figure 4.

Hybridization of three BAC clones in the minimum tiling paths for contigs 1003 and 431 to mitotic squashes (2n = 26) verifies the FPC assemblies. (a-e) Results for contig 1003; (f-j) results for contig 431. Panels (a) and (f) show all three BAC-FISH probes merged; (e,j) DAPI staining; (b,c,d) show each of three BACs (red, green, white) for contig 1003; (g,h,i) show each of three BACs (red, green, white) for contig 431.