Table 1 |
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Commonly used ES cell lines for generating genetically modified mice |
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ES cell line |
Genetic background |
Comments |
Reference |
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E14TG2a |
129P2OlaHsd |
Feeder-independent; suitable for injections into C57BL/6 blastocysts |
[104] |
|
AB2.2 |
129S7/SvEvBrd-Hprtb-m2 |
Feeder-dependent |
[105] |
|
J1 |
129SvJae |
Feeder-dependent |
[106] |
|
Bruce4 |
C57BL/6J-Thy1.1 |
Have a tendency to aneuploidy |
[107] |
|
B6/Blu-1 |
C57BL/6N |
Generated by Tim Ley (Washington University, St Louis, USA) |
Personal communication |
|
JM8.parental |
C57BL/6N |
76% GLT rate |
[31] |
|
JM8.F6 |
C57BL/6N |
Feeder-dependent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts |
[31] |
|
JM8.N4 |
C57BL/6N |
Feeder-independent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts |
[31] |
|
JM8A1.N3 |
C57BL/6N |
JM8.F6-derived line with a repaired Agouti locus. Feeder independent |
[31] |
|
C2 |
C57BL/6NTac |
Efficient GLT using a combination of ICR morula aggregation with outbred host embryos |
[108] |
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R1 |
129X1/SvJ × 129S1 (hybrid) |
Feeder-dependent |
[109] |
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G4 |
129S6/SvEvTac × C57BL/6Ncr F1 hybrid |
Feeder-dependent; typically used for tetraploid complementation assays |
[75] |
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Abbreviations: GLT, germ line transmission; ICR, Institute for Cancer Research. |
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van der Weyden et al. Genome Biology 2011 12:224 doi:10.1186/gb-2011-12-6-224 |
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