Table 1

Commonly used ES cell lines for generating genetically modified mice

ES cell line

Genetic background

Comments

Reference


E14TG2a

129P2OlaHsd

Feeder-independent; suitable for injections into C57BL/6 blastocysts

[104]

AB2.2

129S7/SvEvBrd-Hprtb-m2

Feeder-dependent

[105]

J1

129SvJae

Feeder-dependent

[106]

Bruce4

C57BL/6J-Thy1.1

Have a tendency to aneuploidy

[107]

B6/Blu-1

C57BL/6N

Generated by Tim Ley (Washington University, St Louis, USA)

Personal communication

JM8.parental

C57BL/6N

76% GLT rate

[31]

JM8.F6

C57BL/6N

Feeder-dependent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts

[31]

JM8.N4

C57BL/6N

Feeder-independent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts

[31]

JM8A1.N3

C57BL/6N

JM8.F6-derived line with a repaired Agouti locus. Feeder independent

[31]

C2

C57BL/6NTac

Efficient GLT using a combination of ICR morula aggregation with outbred host embryos

[108]

R1

129X1/SvJ × 129S1 (hybrid)

Feeder-dependent

[109]

G4

129S6/SvEvTac × C57BL/6Ncr F1 hybrid

Feeder-dependent; typically used for tetraploid complementation assays

[75]


Abbreviations: GLT, germ line transmission; ICR, Institute for Cancer Research.

van der Weyden et al. Genome Biology 2011 12:224   doi:10.1186/gb-2011-12-6-224