Additional file 11.
Figure S6 - simulation negative control. (a) Accuracy bar charts and (b) ROC-style curves showing performance of ALL, UNI, and BEST maps on our negative control synthetic DNA template (2 × RPL +0%). Five unknown sample genomes were generated from each reference template by adding SNPs randomly to a proportion of 0.001. Read sets were sampled from each sample genome to one of four coverage levels (25-fold, 50-fold, 100-fold, 200-fold). Read sets were independently aligned to their respective reference genome using ALL, UNI, or BEST maps with k = 1, 2, or 3 mismatches and genotyped using Sniper. (a) Bar charts report genotyping accuracy for each condition. Error bars show ± standard error of the mean over five replicates. (b) ROC-style curves are shown as the number of false positive calls versus sensitivity. Three stringency levels for Q (13, 40, 90) are shown for each curve.
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Simola and Kim Genome Biology 2011 12:R55 doi:10.1186/gb-2011-12-6-r55