Functional amino acid residues are not under-represented in intrinsically disordered regions within proteins. The Limacs functional sites index calculated for mapped Pfam domains within IDRs is plotted against different confidence value thresholds used for prediction of IDRs. The mean fraction of residues predicted to be in IDRs relative to structured regions, at different prediction threshold values, is indicated by open diamonds (default threshold used in the study was 0.8). The corresponding Limacs functional sites index is shown without filtering (filled squares) or after filtering to remove multiple examples of the same Pfam domain (filled circles; see Materials and methods for details).
Nilsson et al. Genome Biology 2011 12:R65 doi:10.1186/gb-2011-12-7-r65