Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Access Research

The minimal kinome of Giardia lamblia illuminates early kinase evolution and unique parasite biology

Gerard Manning1*, David S Reiner234, Tineke Lauwaet2, Michael Dacre1, Alias Smith2, Yufeng Zhai1, Staffan Svard5 and Frances D Gillin2

Author Affiliations

1 Razavi Newman Center for Bioinformatics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA

2 Department of Pathology, University of California at San Diego, 214 Dickinson St CTF-C 415, San Diego, CA 92103-8416, USA

3 Department of Microbiology, Tumor and Cell Biology (MTC), Nobels väg 16, KI Solna Campus, Karolinska Institutet, Box 280, SE-171 77, Stockholm, Sweden

4 Current address: Proveri Inc., 10835 Road to the Cure, Suite 150, San Diego, CA 92121, USA

5 Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, SE-75124, Uppsala, Sweden

For all author emails, please log on.

Genome Biology 2011, 12:R66  doi:10.1186/gb-2011-12-7-r66

Published: 25 July 2011

Abstract

Background

The major human intestinal pathogen Giardia lamblia is a very early branching eukaryote with a minimal genome of broad evolutionary and biological interest.

Results

To explore early kinase evolution and regulation of Giardia biology, we cataloged the kinomes of three sequenced strains. Comparison with published kinomes and those of the excavates Trichomonas vaginalis and Leishmania major shows that Giardia's 80 core kinases constitute the smallest known core kinome of any eukaryote that can be grown in pure culture, reflecting both its early origin and secondary gene loss. Kinase losses in DNA repair, mitochondrial function, transcription, splicing, and stress response reflect this reduced genome, while the presence of other kinases helps define the kinome of the last common eukaryotic ancestor. Immunofluorescence analysis shows abundant phospho-staining in trophozoites, with phosphotyrosine abundant in the nuclei and phosphothreonine and phosphoserine in distinct cytoskeletal organelles. The Nek kinase family has been massively expanded, accounting for 198 of the 278 protein kinases in Giardia. Most Neks are catalytically inactive, have very divergent sequences and undergo extensive duplication and loss between strains. Many Neks are highly induced during development. We localized four catalytically active Neks to distinct parts of the cytoskeleton and one inactive Nek to the cytoplasm.

Conclusions

The reduced kinome of Giardia sheds new light on early kinase evolution, and its highly divergent sequences add to the definition of individual kinase families as well as offering specific drug targets. Giardia's massive Nek expansion may reflect its distinctive lifestyle, biphasic life cycle and complex cytoskeleton.