Single cell transcriptomics of neighboring hyphae of Aspergillus niger
1 Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
2 Microarray Department and Integrative Bioinformatics Unit, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
3 Netherlands Bioinformatics Centre (NBIC), Geert Grooteplein 28, 6525 GA Nijmegen Nijmegen, the Netherlands
Genome Biology 2011, 12:R71 doi:10.1186/gb-2011-12-8-r71Published: 4 August 2011
Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.