Distribution of septa and nuclei in hyphae at the outer part of a sandwiched colony. (a) Calcofluor white staining visualizing the septa within the hyphae (indicated by arrows). The first septum is positioned 400 μm from the apex of the hyphae. (b) GFP fused to H2B visualizing the nuclei. (c) Overlay of (b) with a bright field image. The region 10 to 20 μm from the apex is free from nuclei (indicated by arrows).
de Bekker et al. Genome Biology 2011 12:R71 doi:10.1186/gb-2011-12-8-r71