Table 1 |
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|
Quantitative PCR enrichment measurements from 12 clinical samples |
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|
Percentage |
Parasite [DNA] (pg/μl) |
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|
Sample |
parasite DNA |
WGA |
Pre-hybrid selectiona |
Post-hybrid selectiona |
Fold enrichment |
|
|
|||||
|
Th231.08 (round 1) |
0.11 |
Yes |
1.8 (0.6) |
71.1 (5.6) |
39.7 |
|
Th231.08 (round 2) |
7.7 |
No |
71.1 (5.6) |
349.1 (74.9) |
4.9 |
|
Th145.08 |
20 |
No |
198.4 (17.4) |
477.6 (66.7) |
2.4 |
|
Th032.09 |
12 |
No |
114.7 (2.9) |
372.6 (59.3) |
3.2 |
|
Th029.09 |
3 |
No |
33.6 (0.8) |
317.3 (54.7) |
9.4 |
|
Th093.09 |
2.8 |
No |
28.5 (1.5) |
365.6 (53.4) |
12.8 |
|
Th090.08 |
2.3 |
No |
37.7 (1.1) |
300.4 (46.9) |
8.0 |
|
Th139.08 |
2.1 |
No |
23.6 (0.6) |
346.2 (50.7) |
14.7 |
|
Th197.08 |
1.1 |
No |
14.6 (0.0) |
222.7 (36.1) |
15.3 |
|
Th140.08 |
0.99 |
No |
9.6 (0.1) |
251.5 (37.4) |
26.2 |
|
Th190.08 |
0.64 |
No |
5.1 (0.2) |
218.7 (34.0) |
43.2 |
|
Th238.08 |
0.53 |
No |
6.7 (0.2) |
273.4 (38.1) |
41.0 |
|
Th127.09 |
1.6 |
No |
26.8 (0.4) |
368.5 (57.1) |
13.7 |
|
Th175.08 |
48 |
Yes |
275.8 (7.2) |
556.9 (79.4) |
2.0 |
|
|
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|
aNumbers in parentheses represent standard deviations. WGA, whole genome amplification. |
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|
Melnikov et al. Genome Biology 2011 12:R73 doi:10.1186/gb-2011-12-8-r73 |
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