Table 2 |
|||||
|
Quantitative PCR enrichment measurements |
|||||
|
Hybrid temperature |
Stringency |
Wash |
Pre-hybrid selection [DNA] (pg/μl) |
Post-hybrid selection [DNA] (pg/μl) |
Fold enrichment |
|
|
|||||
|
65°C |
High |
0.10 × SSC |
10.0 |
342.9 |
34.3 |
|
Med/high |
0.25 × SSC |
10.0 |
258.2 |
25.8 |
|
|
Med/low |
0.50 × SSC |
10.0 |
227.9 |
22.8 |
|
|
Low |
0.75 × SSC |
10.0 |
181.4 |
18.1 |
|
|
60°C |
High |
0.10 × SSC |
10.0 |
288.6 |
28.9 |
|
Med/high |
0.25 × SSC |
10.0 |
232.9 |
23.3 |
|
|
Med/low |
0.50 × SSC |
10.0 |
203.5 |
20.4 |
|
|
Low |
0.75 × SSC |
10.0 |
196.3 |
19.6 |
|
|
|
|||||
|
Melnikov et al. Genome Biology 2011 12:R73 doi:10.1186/gb-2011-12-8-r73 |
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