Table 2

Quantitative PCR enrichment measurements

Hybrid temperature

Stringency

Wash

Pre-hybrid selection

[DNA] (pg/μl)

Post-hybrid selection

[DNA] (pg/μl)

Fold enrichment


65°C

High

0.10 × SSC

10.0

342.9

34.3

Med/high

0.25 × SSC

10.0

258.2

25.8

Med/low

0.50 × SSC

10.0

227.9

22.8

Low

0.75 × SSC

10.0

181.4

18.1

60°C

High

0.10 × SSC

10.0

288.6

28.9

Med/high

0.25 × SSC

10.0

232.9

23.3

Med/low

0.50 × SSC

10.0

203.5

20.4

Low

0.75 × SSC

10.0

196.3

19.6


Melnikov et al. Genome Biology 2011 12:R73   doi:10.1186/gb-2011-12-8-r73

Open Data