Figure 3.

Positions of chromosomes 10, 18 and × in HGPS human dermal fibroblasts after FTI treatment (48 hours) and after two passages. Histograms displaying the normalized nuclear positions of chromosome 10, 18 and × territories in interphase nuclei subjected to two-dimensional FISH analysis, and determined by erosion analysis, of untreated AG11498 HGPS fibroblasts (I:a, f, k), AG11498 HDFs treated with equivalent amounts of DMSO used for dissolution of inhibitors (I:b, g, l), AG11498 fibroblasts treated with 2.5 μM FTI-277 (I:c, h, m), AG11498 fibroblasts treated with 2.5 μM GGTI-2147 (I:d, i, n) and AG11498 HDFs treated with a combination of FTI-277 and GGTI-2147 (2.5 μM each) (I:e, j, o). Shell 1 represents the nuclear periphery and shell 5 the nuclear interior. Error bars indicate the standard error of the mean. Filled-in squares indicate statistical difference (P < 0.05) for that shell when compared to the equivalent shell of the untreated sample. Panel II displays the nuclear positioning of cells treated with the inhibitors for 48 hours and then cultured for a further two passages.