Figure 4.

Positions of chromosomes 10, 18 and × in HGPS human dermal fibroblasts after FTI treatment following incubation in low serum. Control fibroblasts and HGPS (AG11513) cells treated with both FTI-277 and GGTI-2147 were placed in low serum, fixed and subjected to two-dimensional FISH and erosion analysis, with shell 1 representing the nuclear periphery and shell 5 the nuclear interior. The histograms display the positions of chromosomes 10, 18 and × in interphase nuclei after 0 (a, e, i), 15 (b, f, j), 30 (c, g, k) minutes and 7 days (d, h, l) following serum withdrawal. Error bars indicate the standard error of the mean. Filled-in squares indicate statistical difference (P < 0.05) for that shell when compared to the equivalent shell for 0 minute sample. The nuclear repositioning of chromosomes 10 and 18 after serum removal [42] was restored in the FTI-treated HGPS cells.