Nuclear myosin 1β staining in control fibroblasts and HGPS fibroblasts before and after FTI treatment. NM1β is a component of the nuclear motor complex that is involved in chromosome movement. Control and HGPS (AG11498) fibroblasts (untreated and treated with FTI and GGTI together) were fixed with 1:1 methanol:acetone and subjected to dual indirect immunofluorescence with commercial rabbit anti-NM1β antibody (a-c) and a mouse anti-pKi67 (to reveal proliferating cells) (d-f). Secondary antibodies were a donkey anti-mouse antibody conjugated to FITC and a swine anti-rabbit antibody conjugated to TRITC. (a) In proliferating control fibroblasts the distribution of NM1β is at the nuclear periphery, in the nucleolus and throughout the nucleoplasm in a fine punctate distribution. (b) In proliferating HGPS fibroblasts slight nucleoplasmic anti-NM1β staining is apparent but staining is mainly distributed as large aggregates near the nucleoli. (c) When HGPS cells were treated with FTI and GGTI together, the distribution of NM1β was restored to the nuclear periphery and throughout the nucleoplasm, although there were still large aggregates present. Scale bar = 10 μm.
Mehta et al. Genome Biology 2011 12:R74 doi:10.1186/gb-2011-12-8-r74