Myosin staining pattern in quiescent HGPS human dermal fibroblasts after FTI treatment and following re-stimulation. AG11498 HDFs treated with FTI-277 were serum starved for 7 days to induce quiescence. The cells were then re-stimulated with fresh serum and samples were collected at 0, 24, 36 and 48 hours after serum restoration. Samples were also collected before serum withdrawal (proliferating cells). The samples were then fixed with methanol:acetone (1:1) and distribution of NM1β was assessed by performing an indirect immunofluorescence assay for NM1β. (a, c, e, g, i) The distribution of NM1β in cells before and after restimulation of quiescent fibroblasts. (b, d, f, h, j) DAPI staining. Scale bar = 10 μm.
Mehta et al. Genome Biology 2011 12:R74 doi:10.1186/gb-2011-12-8-r74