Table 1

Distribution of NM1β in HGPS cells

Proliferating HGPS cells

0 hours (quiescent HGPS cells)

24 hours

36 hours

48 hours


Nucleolar + rim + nucleoplasmic

5.8 ± 3.0

0.8 ± 2.0

2.7 ± 6.24

3.1 ± 3.06

10.4 ± 3.05

Nucleolar only

0 ± 0

19.8 ± 6.65

19.7 ± 6.506

8.0 ± 6.08

8.2 ± 3.05

Aggregates

85.1 ± 6.11

77.3 ± 6.55

71.9 ± 5.85

83.3 ± 3.06

76.4 ± 8.96

Rim + nucleoplasmic

5.2 ± 2.51

0 ± 0

2.2 ± 3.06

2.9 ± 4.0

2.2 ± 3.61

Nucleoplasmic

2.9 ± 2.64

1.4 ± 3.51

1.8 ± 3.51

1.8 ± 4.0

1.8 ± 2.64

Dull

0.1 ± 1.53

0.6 ± 0.57

1.7 ± 3.78

0.7 ± 2.0

0.9 ± 2.51


Proliferating HGPS cells were in 15% serum, quiescent HGPS cells were in 0.5% serum for 7 days, and 24, 36 and 48 hours refer to time after the re-addition of serum. The distribution of NM1β is normally nucleolar + rim + nucleoplasmic in proliferating control cells, while abnormal distributions are nucleolar only, aggregates, rim + nucleoplasmic, nucleoplasmic only and dull. In control quiescent cells aggregates are the most common distribution observed [42]. Numbers are percentage values ± standard deviation. The most prominent distribution of NM1β in HGPS cells in high and low serum was in aggregates.

Mehta et al. Genome Biology 2011 12:R74   doi:10.1186/gb-2011-12-8-r74

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