Table 2

Distribution of NM1β in HGPS cells after a 48 hour FTI treatment

Proliferating HGPS cells + FTI

0 hours (quiescent HGPS cells + FTI)

24 hours

36 hours

48 hours


Nucleolar + rim + nucleoplasmic

73.7 ± 7.09

2.1 ± 1.53

35.0 ± 8.18

51.6 ± 3.61

64.0 ± 4.0

Nucleolar only

0 ± 0

19.3 ± 11.32

14.9 ± 2.0

9.8 ± 3.46

8.5 ± 2.64

Aggregates

18.6 ± 6.55

74.2 ± 5.29

45.8 ± 1.53

31.5 ± 4.58

21.8 ± 2.51

Rim + nucleoplasmic

4.8 ± 2.08

0 ± 0

2.5 ± 1.53

3.8 ± 2.08

1.6 ± 0.06

Nucleoplasmic

1.8 ± 2.08

2.9 ± 2.08

1.2 ± 1.15

2.6 ± 3.21

2.1 ± 2.64

Dull

0.9 ± 1.53

1.5 ± 1.53

0.4 ± 1.73

0.5 ± 2.31

1.9 ± 3.79


Distribution was analyzed in FTI-treated HGPS cells in 15% serum (proliferating), 0.5% serum for 7 days (quiescent) and 24, 36 and 48 hours after the re-addition of serum. The distribution of NM1β in proliferating cells was more like control fibroblasts with a normal distribution of nucleolar + rim + nucleoplasmic that became like normal quiescent cells with the withdrawal of serum and returned to a more normal distribution for proliferating cells within the expected time frame (36 to 48 hours) [42]. Numbers are percentage values with ± standard deviation.

Mehta et al. Genome Biology 2011 12:R74   doi:10.1186/gb-2011-12-8-r74

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