Additional file 3.

Figure S3 - M+1/M isotope ratios. (a, b) The M+1/M isotope ratio 13C enrichment of (a) glutamate and (b) isocitrate analyzed by GC-MS of the aqueous fraction from control and PPARγ agonist-treated 3T3-L1 cells incubated with 13C-U-palmitate. (c-e) Graphs showing the isotope ratio 13C enrichment of myristate (c), arachidate (d) and palmitate (e) analyzed by GC-MS of the organic fraction from control and PPARγ agonist-treated 3T3-L1 cells incubated with 13C-U-palmitate. The metabolites have been mapped to the TCA cycle and fatty acid β-oxidation/synthesis metabolic pathways. Red indicates a metabolite increased in 13C enrichment by PPARγ activation. Blue indicates a metabolite decreased in 13C enrichment by PPARγ activation. *P < 0.05, **P < 0.01,***P < 0.005. Parent ions were used to calculate ion ratio.

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Roberts et al. Genome Biology 2011 12:R75   doi:10.1186/gb-2011-12-8-r75