Next generation sequencing reveals the planarian head regeneration transcriptome. (a) Schematic overview of the two-step amputation and sample collection approach. (b) Schematic overview of the regeneration time course. Individual samples are indicated as red dots, which were analyzed in five pooled sequencing libraries (black lines, A to E). Two independent control samples were taken immediately after amputation (green dots). (c, d) Examples of Illumina transcriptome sequencing (RNAseq) reads mapped with Tophat to regions on genomic supercontig v31.000002. The short read coverage, calculated from reads from all sequenced samples, is shown in black. Green gene models represent MAKER predictions; blue models exemplify the results of the de novo assembly (Illumina+).
Sandmann et al. Genome Biology 2011 12:R76 doi:10.1186/gb-2011-12-8-r76