Additional file 1.

Transcriptome assembly from public 454 data. (a-c) Schematic overview of 454 transcriptome assembly approaches. Publicly available 454 reads were assembled either separately (a, b) or as a combined dataset (c) using the Newbler 2.5 assembler. Colors indicate the three different assemblies: yellow, Abril et al. [17] (a); orange Blythe et al. [18] (b); red, combined (c). Quality metrics shown include the shortest and longest sequences in each assembly, as well as N50, for which 50% of all bases are contained in sequences at least as long as N50. (d) Kernel densities of the length distributions for the assembled sequences. For multi-isoform loci, only the longest isoform was considered. Colors as in (a-c). (e) Kernel densities of ortholog hit ratios obtained by comparing sequences from the different assemblies or computational prediction to the Schistosoma mansoni proteome using blastx. For multi-isoform loci, only the longest isoform was considered. Colors as in (a-c).

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Sandmann et al. Genome Biology 2011 12:R76   doi:10.1186/gb-2011-12-8-r76