Figure 4.

Properties of Argonaute interaction site generation and their comparison to crosslink-centered regions. (a) Distribution of interaction site sizes for the Argonaute dataset for sites that fall within 3' UTRs and contain two or more T = > C conversion locations. The vertical red line represents the 41-nucleotide size of the Hafner et al. [7] crosslink-centered regions (CCRs). (b) Distribution of interaction site locations across a normalized 3' UTR for all clusters that have two or more T = > C conversion locations. (c) The signal-to-noise for the top 20 expressed miRNAs in the Argonaute dataset for both PARalyzer generated interaction sites and the Hafner et al. [7] CCRs located in 3' UTRs. (d) Average log2 signal-to-noise ratio of window size 21 across all 361 miRNAs reported expressed in Hafner et al. in the order of their expression rank.