Ratio of read coverage at intra-species variable sites. (a) Density distributions of log2 ratio of read coverage at IVSs. The log2 ratio of the coverage depth was calculated by dividing the number of reads harboring a variant similar to the reference sequence by the number of reads harboring an alternative variant. True and false IVSs correspond to variable sites confirmed or non-confirmed, respectively, by Sanger sequencing. (b) The distribution of log2 coverage ratio at all variable sites detected by mapping sequence capture data to the reference sequence.
Saintenac et al. Genome Biology 2011 12:R88 doi:10.1186/gb-2011-12-9-r88