Sharing of single nucleotide variants between the exome capture kits. The number of all sequenced variants in the common target region was specified as the combination of all variants found with a minimum coverage of 20× in any of the exome capture kits (altogether, 15,044 variants). Variable positions were then examined for sharing between all kits, both Agilent kits, both NimbleGen kits, Agilent SureSelect kit and NimbleGen SeqCap kit, and Agilent SureSelect 50 Mb kit and NimbleGen SeqCap v2.0 kit. Numbers for the shared variants between the kits in question are given, followed by the number of shared variants with the same genotype calls. The diagram is schematic, as the sharing between Agilent SureSelect and NimbleGen SeqCap v2.0, Agilent SureSelect 50 Mb and NimbleGen SeqCap or any of the combinations of three exome capture kits is not illustrated.
Sulonen et al. Genome Biology 2011 12:R94 doi:10.1186/gb-2011-12-9-r94