Figure 4.

Visualization of the p.Arg166His mutation in E2F1. Top: the E2F4 crystal structure [PDB:1CF7] showing the location of the p.Arg166His mutation. The brown double helix is the DNA binding motif with green colored guanine nucleotides representing binding targets of Arg182 and Arg183 of the DP2 protein and yellow colored guanine nucleotides representing binding targets of Arg166 and Arg165 of the E2F protein. The blue ribbon represents the DNA binding region of E2F with the Arg166 mutation in red and Arg165 in blue, while the purple ribbon represents the DNA binding region of DP2 with Arg182 and Arg183 in purple. Bottom: a schematic showing binding of E2F residues to DNA binding site nucleotides.