Figure 6.

Mutation of R166 in E2F1 affects its efficiency of binding to promoter targets. (a) ChIP assay on MSTO-211H cells transiently transfected with E2F1 wild type (WT) or E2F1-R166H (R166H) for 48 hours using anti-Myc antibody. The amplification levels of the APAF1 (top) and SIRT1 (bottom) promoters were determined by PCR. Anti-IgG antibody was used as negative control. (b, c) Expression levels of E2F1 targets - SIRT1, APAF1, and CCNE1 - in MSTO-211H and NCI-H28 cells that were transfected with the indicated plasmids. Each bar represents mean ± standard deviation (n = 3; *P < 0.05, **P < 0.01). Ctrl, empty vector.