Figure 7.

Accumulation of mutant E2F1 protein in cells due to increased stability of E2F1-R166H. (a) E2F1 protein levels detected by anti-E2F1 antibody (KH95) 48 hours after transfection. WT, wild type. (b) Degradation assay performed in MSTO-211H cells over-expressing E2F1 treated with 25 μg/ml cycloheximide. Levels of E2F1 protein were monitored every 30 minutes for 3 hours using anti-E2F1 antibody.