Table 1

Human DNA samples and exome captures used in this study

Number of captures


Our ID

Population

HapMap ID

Family member

NimbleGen

Agilent


CEU-M

CEU

NA12892

Mother

1

0

CEU-F

CEU

NA12891

Father

1

0

CEU-D

CEU

NA12878

Daughter

1

0

YRI-M

YRI

NA19238

Mother

4

4

YRI-F

YRI

NA19239

Father

2

0

YRI-D

YRI

NA19240

Daughter

4

4


The human genomic DNA samples used for this study were chosen to match those analyzed by the trio pilot of the 1000 Genomes Pilot [13]. The sources of the genetic material consist of a European ancestry in Utah, USA population (CEU) trio and a Yoruba in Ibadan, Nigeria population (YRI) trio, with each trio composed of a mother, a father, and a daughter. The table reviews the exome captures performed with each sample. For DNA samples from which multiple exome captures were performed, independent libraries were prepared from each DNA sample in order to support independent exome captures. Exome captures were performed with either the SeqCap EZ Exome Library SR (NimbleGen) or the SureSelect Human All Exon Kit (Agilent), which were designed to capture well-annotated protein-coding regions of the human hg18 (NCBI36.1) assembly. Following exome capture, each captured library was sequenced using paired-end 76-cycle chemistry, in one flowcell lane on the Genome AnalyzerIIx instrument (Illumina).

Parla et al. Genome Biology 2011 12:R97   doi:10.1186/gb-2011-12-9-r97

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