Ambiguities in RNA-Seq data. This figure demonstrates ambiguities that arise in RNA-Seq data that make isoform prediction challenging. Because there is read coverage across several introns, SpliceGrapher is not able to determine whether this is a result of a single intron retention event, or several independent events.
Rogers et al. Genome Biology 2012 13:R4 doi:10.1186/gb-2012-13-1-r4