A total of 106, 75 bp long reads were simulated from human (NCBI37) genomes. Eleven data sets with different rates of bisulfite conversion, 0% to 100% at increments of 10% (context is indicated), were created and aligned to the NCBI37 genome. (a-e) The x-axis represents the detected methylation conversion percentage. The y-axis represents the simulated methylation conversion percentage. (f) The x-axis represents the mapping efficiency of the programs. The y-axis represents the simulated methylation conversion percentage of the data set that the program is mapping. (a,b) The mapping statistics for various genomic contexts and mapping efficiency with data sets at different rates of bisulfite conversion for BatMeth and B-SOLANA, respectively. (c-e) Comparison of the methylated levels detected by BatMeth and B-SOLANA in the context of genomic CG, CHG and CHH, respectively. (f) Comparison of mapping efficiencies of BatMeth and B-SOLANA across data sets with the described various methylation levels.
Lim et al. Genome Biology 2012 13:R82 doi:10.1186/gb-2012-13-10-r82