Acetylated-H2A.Z localizes to bivalent promoters. (a) Heatmaps depict acH2A.Z ChIP-Seq signals at transcription start sites (TSSs) (±5 kb), organized according to their chromatin status: H3K4me3 (green), H3K27me3 (red), bivalent (green and red), and no-mark (white) in mES cells and mNP. acH2A.Z is enriched at promoters in both cell types, but is depleted from monovalent K27me3-only promoters. (b) ChIP-Seq tracks show acH2A.Z localization to K4me3-only (Pol2rd) and bivalent promoters (St8sia4 and Ihh) in mES cells. (c) Composite plot shows acH2A.Z ChIP-Seq signal is enriched at H2A.Z-enriched TSS (±5 kb). (d) Analogous composite plots of acH2A.Z at H2A.Z-enriched intergenic sites (±5 kb). (e) Composite plot shows H2A.Z ChIP-Seq signal around TSSs (±2.5 kb), segregated by transcription quartile levels in mES cells. (f) Analogous composite plots for acH2A.Z ChIP-Seq signals. ac: acetylated; ChIP-Seq: chromatin immunoprecipitation coupled with high-throughput sequencing; K: lysine; kb: kilobases; me3: trimethylation; mES: mouse embryonic stem; mNP: mouse neural progenitor cell; TSSs: transcription start sites.
Ku et al. Genome Biology 2012 13:R85 doi:10.1186/gb-2012-13-10-r85