This article is part of a special issue on epigenomics.

Open Access Method

Enhanced top-down characterization of histone post-translational modifications

Zhixin Tian12, Nikola Tolić1, Rui Zhao1, Ronald J Moore3, Shawna M Hengel1, Errol W Robinson1, David L Stenoien3, Si Wu1, Richard D Smith3 and Ljiljana Paša-Tolić1*

Author Affiliations

1 Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99352, USA

2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, Liaoning 116023, China

3 Biological Sciences Division, Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99352, USA

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Genome Biology 2012, 13:R86  doi:10.1186/gb-2012-13-10-r86

Published: 3 October 2012

Additional files

Additional file 1:

Additional information regarding histone H4 tandem MS results. Data are contained in five sheets in a Microsoft Excel file format. Sheet one contains a collection of information from the data analysis software. Sheet two contains protein isoform information specific to ETD fragmentation. Sheet three contains protein isoform information specific to CID fragmentation. Sheet four summarizes protein isoform information common to both CID and ETD. Sheet five summarizes ETD and CID fragment ions. CID, collision induced dissociation; ETD, electron transfer dissociation; MS, mass spectrometry.

Format: XLS Size: 213KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Additional information regarding histone 2B tandem MS results. Data are contained in three sheets in a Microsoft Excel file format. Sheet one contains a summary of information from the data analysis software. Sheet two contains protein isoform information specific to CID fragmentation. Sheet three contains protein isoform information specific to ETD fragmentation. CID, collision induced dissociation; ETD, electron transfer dissociation; MS, mass spectrometry.

Format: XLS Size: 183KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Additional information regarding histone 2A tandem MS results. Data are contained in three sheets in a Microsoft Excel file format. Sheet one contains a summary of information from the data analysis software. Sheet two contains protein isoform information specific to ETD fragmentation. Sheet three contains protein isoform information specific to CID fragmentation. CID, collision induced dissociation; ETD, electron transfer dissociation; MS, mass spectrometry.

Format: XLS Size: 134KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Additional information regarding histone 3 first fraction tandem MS results. Data are contained in three sheets in a Microsoft Excel file format. Sheet one contains a summary of information from the data analysis software. Sheet two contains protein isoform information specific to CID fragmentation. Sheet three contains protein isoform information specific to ETD fragmentation. CID, collision induced dissociation; ETD, electron transfer dissociation; MS, mass spectrometry.

Format: XLS Size: 344KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 5:

Additional information regarding histone 3 second fraction tandem MS results. Data are contained in three sheets in a Microsoft Excel file format. Sheet one contains a summary of information from the data analysis software. Sheet two contains protein isoform information specific to ETD fragmentation. Sheet three contains protein isoform information specific to CID fragmentation. CID, collision induced dissociation; ETD, electron transfer dissociation; MS, mass spectrometry.

Format: XLS Size: 300KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 6:

Additional information regarding histones from a single RPLC separation. Data are contained in a Microsoft Excel file format and present a summary of information from the data analysis software for the results from a single dimension RPLC separation. RPLC, reversed phase liquid chromatography.

Format: XLS Size: 147KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 7:

Additional information regarding histones from a single WCX-HILIC separation. Data are contained in a Microsoft Excel file format and present a summary of information from the data analysis software for the results from a single dimension WCX-HILIC separation. WCX-HILIC, weak cation exchange-hydrophilic interaction liquid chromatography.

Format: XLS Size: 170KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 8:

Schematic diagram of the online metal-free RPLC/WCX-HILIC system. Details of the experimental set-up used to perform the described HPLC separations, including valve and flow path details. HPLC, high performance liquid chromatography; RPLC/WCX-HILIC, reversed phase liquid chromatography-weak cation exchange-hydrophilic interaction liquid chromatography.

Format: DOCX Size: 68KB Download file

Open Data