Increase of detection dynamic range with 2D separation. Representative mass spectra (charge state 14+) for chromatographic peaks shown in Figure 1. (a) Isoforms of H4 observed from RPLC separation of HeLa core histones; (b to f) isoforms of H4 observed from 2D RP/WCX-HILIC separation of histone H4. Isoforms identified from the most abundant peaks as marked with the dotted lines together with P scores (top, RPLC; bottom: WCX-HILIC) are noted above (b). RPLC, reversed phase liquid chromatography; WCX-HILIC, weak cation exchange-hydrophilic interaction liquid chromatography.
Tian et al. Genome Biology 2012 13:R86 doi:10.1186/gb-2012-13-10-r86