Table 2

Comparison of CID and ETD for histone isoform identification using 2D RP-WCX-HILIC LC-MS/MS analysis.

H4

H2B

H2A

H3

Total

Subtotal

105 (10)

110 (19)

77 (28)

416 (69)

708 (126)

CID

48 (5)

57 (17)

9 (4)

44 (8)

158 (34)

ETD

57 (5)

53 (2)

68 (24)

372 (61)

550 (92)

The total numbers of isoforms identified by either CID or ETD are shown with phosphorylated isoforms in parentheses. CID, collision induced dissociation; ETD, electron transfer dissociation; 2D RP-WCX-HILIC LC-MS/MS, two dimensional reverse phase-weak cation exchange-hydrophilic interaction liquid chromatography-tandem mass spectrometry.

Tian et al. Genome Biology 2012 13:R86   doi:10.1186/gb-2012-13-10-r86