Figure 1.

Flowchart comparing RRBS and mRRBS steps. Each step that can be completed in a standard workday is shown. Orange boxes highlight phenol:chloroform clean-up and preparative agarose gel purification steps that were omitted in the new mRRBS protocol. Purple boxes highlight key new steps specific to mRRBS. Each box also shows the approximate amount of hands-on time required per step. QC, quality control.