Table 1

Summary of mRRBS performance

Description

Total reads

Informative reads

Bisulfite conversion

1× coverage CpG count

5× coverage CpG count

10× coverage CpG count


96 samples

11,295,879

8,921,543

99%

2,523,793

1,399,192

563,980

84 HQ samples

12,151,833

9,629,839

99%

2,583,636

1,510,414

645,828


The first row summarizes statistics for all 96 libraries generated using mRRBS, and the second row includes only those high-quality (HQ) samples with greater than 5 million reads per sample (see Additional file 2 for per-sample details). The total reads column gives the median number of sequencing reads produced for each library. The number of those reads that passed sequencer quality controls, were aligned to the reference genome, and included in the informative read count (median value). The estimated bisulfite conversion rate is based on all methylated cytosines in a non-CpG context [6]. The median numbers of CpGs covered with at least 1×, 5×, and 10× coverage is shown.

Boyle et al. Genome Biology 2012 13:R92   doi:10.1186/gb-2012-13-10-r92

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