Table 3

Cost comparison of RRBS and mRRBS

mRRBS

RRBS


Enzymes

Total (96 samples) enzymes

$665.99

$998.69

Per sample

$6.94

$10.40

Other supplies and sequencinga

Total (96 samples) other supplies and sequencing

$16,770.00

$15,360.00

Per sample

$174.69

$160.00

With salaryb

Total (96 samples) supplies + salary

$18,820.60

$37,254.08

Total per sample cost

$196.05

$388.06


aSequencing costs are based on the current list price for HiSeq 40 bp indexed single read at the Whitehead Institute Core Facility. bUsing the previous RRBS method, an estimated 72 days are required to complete library preparation for 96 samples, whereas only 6 days are required using the mRRBS method. Salary costs are calculated using these labor estimates with a $60,000 annual research associate salary. Values are US dollars.

Boyle et al. Genome Biology 2012 13:R92   doi:10.1186/gb-2012-13-10-r92

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