Additional file 2.

Figure S2. qPCR analysis on pre- and post-whole genome amplification (WGA) material introduced no bias upon amplification. Confirmatory qPCR showing hmC enrichment at Tex19.1 and H19 promoter regions (known hmC-enriched loci) alongside a 5hmC-negative control region (Gapdh promoter) both prior to and after WGA. 5mC enrichment was tested in a similar way using positive control regions of the H19 ICR and Cyp2b10 promoter regions and a negative ICR.

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Thomson et al. Genome Biology 2012 13:R93   doi:10.1186/gb-2012-13-10-r93