Additional file 5.

Figure S5. Validation of 'EpiMark' quantitative analysis techniques reveals highly similar levels of the DNA modifications between individuals. (a-c) The values of 5hmCpG (a), 5mCpG (b) and CpG (c)were plotted between two biological replicates to test the reproducibility of the result. Loci tested were as follows: TSS regions of Gapdh, H19 and Tspan10, an intra-genic region of Gstm3, a region upstream of the Cyp2b10 promoter and an inter-genic region spanning chr7: 149709621-149709863 (see primer list in Additional file 25). All modified forms of CpG are reproducible between individuals over the six loci. Pearson correlation values ('cor') are shown alongside representing the reproducibility of results.

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Thomson et al. Genome Biology 2012 13:R93   doi:10.1186/gb-2012-13-10-r93