Additional file 4.

Supplemental figure depicting bisulfite-PCR sequence analysis. (a) Contig4414.0 following bisulfite treatment of 40 h DNA and PCR with C-to-T converted primers, which are not methyl-specific. (b) Contig4414.0 following bisulfite treatment of 40 h DNA and PCR with cytosine-retaining (methyl-specific) primers. (c) The 170 bp satellite following bisulfite treatment of vegetative and PCR with C-to-T converted primers, which are not methyl-specific. (d) The 170 bp satellite following bisulfite treatment of 40 h DNA and PCR with cytosine-retaining (methyl-specific) primers. (e) The transposon TBE1 following bisulfite treatment of 40 h DNA and PCR with cytosine-retaining (methyl-specific) primers. TBE1, telomere bearing element 1.

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Bracht et al. Genome Biology 2012 13:R99   doi:10.1186/gb-2012-13-10-r99