Clustering, microbial community composition and microbial diversity of samples from different sampling sites. Composition is displayed as relative abundance, that is, the number of reads assigned to a genus divided by the total number of reads assigned up to the genus level. (a) Clustering of 20 samples of five healthy subjects. Samples were clustered using UPGMA with weighted UniFrac as a distance measure. The figure was generated with the interactive Tree of Life tool (iTOL) . Participating volunteers are numbered HV1 to HV5 followed by the sampled body location. Colored bars represent the relative abundance of bacterial genera as determined by barcoded pyrosequencing (details in Materials and methods). (b) Phylogenetic diversity rarefaction curves for communities sampled from the listed skin locations show differences between armpit, forehead, inner elbow and upper buttock skin.
Zeeuwen et al. Genome Biology 2012 13:R101 doi:10.1186/gb-2012-13-11-r101