Additional file 3.

Experimental set-up tape-stripping upper buttock skin. On the right site the experiment is depicted that was used to study the human skin microbiome in different epidermal layers. Two areas on the right upper buttock measuring 2 cm2 (1 × 2 cm) each, were tape-stripped 5 (STR5) and 10 (STR10) times respectively by application and removal of adhesive tape (n = 12, F1-6 and M1-6). Subsequently, barrier disrupted skin areas were sampled, as well as 2 cm2 healthy non-barrier disrupted right upper buttock skin (STR0). To study recolonization of the human skin microbiome after skin barrier disruption, four areas on the left upper buttock measuring 2 cm2 each, were tape-stripped 15 times (n = 6, F1-3 and M1-3) or as many times as required to obtain a glistening surface which indicates complete removal of the stratum corneum (F4-6 and M4-6).

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Zeeuwen et al. Genome Biology 2012 13:R101   doi:10.1186/gb-2012-13-11-r101