Additional file 9.

Yield bacterial genomic DNA after tape-stripping. Swabs were taken from upper buttock skin and from skin that was tape-stripped 1, 10, 15, and 30 times on this part of the body. Genomic DNA was extracted using the Mobio Ultraclean Microbial DNA Isolation Kit and concentrations were determined by real-time qPCR using broad range universal primers targeting the 16S rRNA gene [71] and calculated from a standard dilution series of Staphylococcus epidermidis.

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Zeeuwen et al. Genome Biology 2012 13:R101   doi:10.1186/gb-2012-13-11-r101