Figure 8.

Human genes with CpG promoters show distinct transcription-coupled changes in chromatin. (a) Multiple transcription elongation-associated histone modifications are detected on nucleosomes closer to the transcription start site (TSS) in genes with CpG islands in their promoters (orange) compared to other genes (green). (b) In contrast, other transcription-associated histone modifications are excluded from a promoter proximal region. (c) H3K36me1 is only detected in the bodies of genes with CpG promoters. (d) Additional transcription-associated modifications are enriched in CpG promoter genes and shift in location away from the start as expression levels increase. (e) Taken together, this means that genes with CpG promoters show a characteristic series of modified nucleosomes upstream, downstream, and at the transcription start site.