Table 1 |
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Predicted knockout strategies for Astaxanthin over-production in cyanobacteria |
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Organism |
Growth rate (mutant/wt) [h-1] |
Astaxanthin production [μmol gDW-1 h-1] (min/max) |
Knockout reaction names |
KEGG reaction ID/EC number |
|
|
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P. marinus as9601 |
0.026/0.061 |
2.18/2.18 |
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
|
5-O-(1-Carboxyvinyl)-3-phosphoshikimate phosphate-lyase |
R01714/EC 4.2.3.5 |
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|
T. elongatus |
0.016/0.048 |
2.08/2.08 |
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
|
Phosphoenolpyruvate: D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase |
R01826/EC 2.5.1.54 |
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|
G. violaceus |
0.033/0.061 |
1.64/1.64 |
Succinate:(acceptor) oxidoreductase |
R00408/EC 1.3.99.1 |
|
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
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|
Synechococcus pcc7942 |
0.033/0.061 |
1.64/1.64 |
Succinate:(acceptor) oxidoreductase |
R00408/EC 1.3.99.1 |
|
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
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|
Cyanobacteria cyb |
0.032/0.058 |
1.57/1.57 |
Succinate:(acceptor) oxidoreductase |
R00408/EC 1.3.99.1 |
|
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
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|
T. erythraeum |
0.051/0.061 |
0.24/0.24 |
Hydrogen-carbonate: L-glutamineamido-ligase |
R00575/EC 6.3.5.5 |
|
Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase |
R01658/EC 2.5.1.1 |
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|
A. variabilis |
0.051/0.061 |
0/0.96 |
4-Methyl-2-oxopentanoate: NAD+ oxidoreductase |
R01651/EC 1.2.1.25 |
|
1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase |
R05636/EC 2.2.1.7 |
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|
Anabaena |
0.051/0.061 |
0/0.96 |
3-(4-Methylpent-3-en-1-yl)-pent-2-enedioyl-CoA hydrolyase |
R03493/EC 4.2.1.57 |
|
1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase |
R05636/EC 2.2.1.7 |
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|
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For each species the table shows the predicted reactions whose knockout is expected to provide maximal astaxanthin production rate, the expected astaxanthin production rate, and the expected decline in growth rate. |
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Vitkin and Shlomi Genome Biology 2012 13:R111 doi:10.1186/gb-2012-13-11-r111 |
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