Table 1

Predicted knockout strategies for Astaxanthin over-production in cyanobacteria

Organism

Growth rate (mutant/wt) [h-1]

Astaxanthin production [╬╝mol gDW-1 h-1] (min/max)

Knockout reaction names

KEGG reaction ID/EC number


P. marinus as9601

0.026/0.061

2.18/2.18

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

5-O-(1-Carboxyvinyl)-3-phosphoshikimate phosphate-lyase

R01714/EC 4.2.3.5

T. elongatus

0.016/0.048

2.08/2.08

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

Phosphoenolpyruvate: D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase

R01826/EC 2.5.1.54

G. violaceus

0.033/0.061

1.64/1.64

Succinate:(acceptor) oxidoreductase

R00408/EC 1.3.99.1

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

Synechococcus pcc7942

0.033/0.061

1.64/1.64

Succinate:(acceptor) oxidoreductase

R00408/EC 1.3.99.1

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

Cyanobacteria cyb

0.032/0.058

1.57/1.57

Succinate:(acceptor) oxidoreductase

R00408/EC 1.3.99.1

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

T. erythraeum

0.051/0.061

0.24/0.24

Hydrogen-carbonate: L-glutamineamido-ligase

R00575/EC 6.3.5.5

Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase

R01658/EC 2.5.1.1

A. variabilis

0.051/0.061

0/0.96

4-Methyl-2-oxopentanoate: NAD+ oxidoreductase

R01651/EC 1.2.1.25

1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase

R05636/EC 2.2.1.7

Anabaena

0.051/0.061

0/0.96

3-(4-Methylpent-3-en-1-yl)-pent-2-enedioyl-CoA hydrolyase

R03493/EC 4.2.1.57

1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase

R05636/EC 2.2.1.7


For each species the table shows the predicted reactions whose knockout is expected to provide maximal astaxanthin production rate, the expected astaxanthin production rate, and the expected decline in growth rate.

Vitkin and Shlomi Genome Biology 2012 13:R111   doi:10.1186/gb-2012-13-11-r111

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