Table 3

FOCAL designed experiments for reactions in galacturonate and glucuronate catabolism

Chosen reaction^a

Enzyme

Associated gene

FOCAL selected carbon source

galur tagur

D-Galacturonate isomerase

uxaC

D-Galacturonate

h + nadh + tagur altrn + nad

Altronate oxidoreductase

uxaB

D-Galacturonate

altrn → 2ddglcn + h₂o

Altronate hydrolase

uxaA

D-Galacturonate

glcur fruur

D-Glucuronate isomerase

uxaC

D-Glucuronate

fruur + h + nadh mana + nad

D-Mannonate oxidoreductase

uxuB

D-Glucuronate

mana → 2ddglcn + h₂o

D-Mannonate hydrolyase

uxuA

D-Glucuronate

^aAbbreviations match those shown in Figure 5.

Tervo and Reed Genome Biology 2012 13:R116   doi:10.1186/gb-2012-13-12-r116