Predicted targets of miR-450a and ocular disease genes are disjoint, yet functionally associated. (a) Targets of miR-450a (DUSP10, AMD1, ZNF385A, colored nodes) and genes associated with macular dystrophy (ELOVL4, BEST1, PRPH2, black nodes) are 'close' to each other in the human functional network (false discovery rate (FDR) ≤8.54e-3). There are no shared genes and direct connections are indicated by bold edges. (b) Northern analysis of mouse eye total RNA suggests miR-450a is active in eye development during embryonic stages. U6 spliceosomal RNA serves as a loading control. (c) In contrast to miR-450a, predicted targets Dusp10, Amd1, and Zfp385a (mouse homolog of ZNF385A) are expressed at low levels during early stages.
Wang et al. Genome Biology 2012 13:R125 doi:10.1186/gb-2012-13-12-r125