Research
Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence
1 Université de Perpignan Via Domitia, CNRS, UMR 5244 Ecologie et Evolution des Interactions (2EI), 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France
2 Department of Biochemistry and Biophysics, ALS 2011, Oregon State University, Corvallis, OR 97331-7305, USA
3 CEBio - Centro de Excelência em Bioinformática, Rua Araguari, 741/301 - Barro Preto - BH/MG - CEP 30190-110, Brazil
Genome Biology 2012, 13:R14 doi:10.1186/gb-2012-13-2-r14
Published: 29 February 2012Additional files
Additional file 1:
List of male-specific scaffolds with putative genes.
Format: DOC Size: 192KB Download file
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Additional file 2:
Video of adult schistosomes treated with TSA at 100 µM. Individuals were filmed with a conventional numerical camera adapted to a stereomicroscope after 5 hours of treatment.
Format: M4V Size: 473KB Download file
Additional file 3:
Video of adult mock-treated schistosomes. Individuals were filmed with a conventional numerical camera adapted to a stereomicroscope after 5 hours of treatment.
Format: M4V Size: 437KB Download file
Additional file 4:
Photographs of ethidium bromide stained PCR products after migration through 2% agarose gels. PCR amplification was used to confirm size and sex-specificity of assembled W-specific repeats. Genomic DNA of two female (F1, F2) and two male individuals (M1, M2) was used as template.
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Additional file 5:
Primers used in this study.
Format: DOC Size: 78KB Download file
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