Open Access Research

Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence

Julie MJ Lepesant1*, Céline Cosseau1, Jérome Boissier1, Michael Freitag2, Julien Portela1, Déborah Climent1, Cécile Perrin1, Adhemar Zerlotini3 and Christoph Grunau1

Author Affiliations

1 Université de Perpignan Via Domitia, CNRS, UMR 5244 Ecologie et Evolution des Interactions (2EI), 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France

2 Department of Biochemistry and Biophysics, ALS 2011, Oregon State University, Corvallis, OR 97331-7305, USA

3 CEBio - Centro de Excelência em Bioinformática, Rua Araguari, 741/301 - Barro Preto - BH/MG - CEP 30190-110, Brazil

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Genome Biology 2012, 13:R14  doi:10.1186/gb-2012-13-2-r14

Published: 29 February 2012

Additional files

Additional file 1:

List of male-specific scaffolds with putative genes.

Format: DOC Size: 192KB Download file

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Open Data

Additional file 2:

Video of adult schistosomes treated with TSA at 100 µM. Individuals were filmed with a conventional numerical camera adapted to a stereomicroscope after 5 hours of treatment.

Format: M4V Size: 473KB Download file

Open Data

Additional file 3:

Video of adult mock-treated schistosomes. Individuals were filmed with a conventional numerical camera adapted to a stereomicroscope after 5 hours of treatment.

Format: M4V Size: 437KB Download file

Open Data

Additional file 4:

Photographs of ethidium bromide stained PCR products after migration through 2% agarose gels. PCR amplification was used to confirm size and sex-specificity of assembled W-specific repeats. Genomic DNA of two female (F1, F2) and two male individuals (M1, M2) was used as template.

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Open Data

Additional file 5:

Primers used in this study.

Format: DOC Size: 78KB Download file

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Open Data