Table 2 |
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W-chromosome-specific repeats of S. mansoni |
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GenBank accession number |
Name |
Length (bp) |
Percentage female hits |
Repeat familya |
Transcription evidence |
FISH localization |
Copy number estimateb |
Reference |
|
|
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|
SMAlphafem-1 |
338 |
99.86 |
SMAlpha retroposon |
RT-PCR |
p-arm |
60,000 - 70,000 |
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|
W1 |
482 |
100 |
Retro |
Middle of q-arm at frontier between heterochromatin and euchromatin as satellite, middle of q-arm |
500 |
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|
W2 |
715 |
100 |
400 |
[62] |
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|
W3 |
786 |
100 |
LTR, highly similar to W2, highly similar to R = 407 |
No transcription (RT-PCR) |
As satellite in the middle of q-arm at frontier between heterochromatin and euchromatin or also in the pericentromeric region |
200 |
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|
W4 |
1132 |
99.52 |
Highly similar to R = 879 |
RT-PCR |
Same location as W1 |
800 |
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|
W5 |
1129 |
99.27 |
LTR, similar to Perere-2, identical to R = 564 |
EST and RT-PCR |
Either at the frontier of heterochromatin and euchromatin of the q-arm or in the pericentromeric region, or at both locations |
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|
W6 |
310 |
99.88 |
Retro |
EST |
In the pericentromeric region |
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|
W7 |
1000 |
100 |
DNA transposon, 97% identical to GenBank accession number XP_002570219 (hypothetical protein Smp_186230) |
In the pericentromeric region |
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|
W8 |
266 |
99.97 |
Tandem repeat (previously described as TR266), DNA transposon |
Either at the frontier of heterochromatin and euchromatin of the q-arm or in the pericentromeric region |
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|
W9 |
803 |
100 |
LINE2, similar to SjR2 retrotransposon |
EST |
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|
W10 |
682 |
100 |
LTR |
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|
W11 |
376 |
100 |
LINE, similar to R = 170 |
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|
W12 |
264 |
100 |
Retro, 97 to 100% identical to several hypothetical S.m. proteins |
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|
W13 |
258 |
100 |
Retro |
In the middle of the heterochromatic part of the q-arm as satellite |
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|
W14 |
209 |
100 |
DNA transposon, similar to R = 170 |
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|
W15 |
185 |
96.62 |
DNA transposon |
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|
W16 |
164 |
100 |
Similar to R = 116 |
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|
W17 |
160 |
100 |
LTR |
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|
W18 |
160 |
100 |
LTR |
EST |
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|
W19 |
139 |
100 |
Retro, 100% identical to GenBank accession number XP_002569391 (hypothetical protein Smp_181820) |
EST |
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|
W20 |
138 |
100 |
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|
W21 |
138 |
99.84 |
DNA transposon, similar to R = 116 |
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|
W22 |
132 |
100 |
DNA transposon |
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|
W23 |
125 |
100 |
DNA transposon |
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|
W24 |
115 |
99.56 |
Retro, similar to Sh122 repeat and R = 31 |
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|
W25 |
112 |
99.65 |
LTR |
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|
W26 |
111 |
100 |
DNA transposon |
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|
W27 |
110 |
100 |
DNA transposon, similar to R = 133 and Sh microsatellite C2 |
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|
W28 |
108 |
100 |
Similar to Sh microsatellite C140 |
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|
W29 |
97 |
100 |
Similar to Sb Sbov20 repeat |
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|
W30 |
96 |
99.05 |
LTR |
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|
W31 |
92 |
100 |
LTR |
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|
W32 |
89 |
96.88 |
DNA transposon |
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|
W33 |
86 |
99.86 |
LTR |
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|
W34 |
82 |
100 |
DNA transposon |
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|
W35 |
80 |
100 |
Retro |
EST |
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|
|
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|
Because of the WZ-type chromosome set of females, these repeats are female-specific. aCombined Censor, Blast, Teclass results. bqPCR based. LINE, long interspersed element; LTR, long terminal repeat. |
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|
Lepesant et al. Genome Biology 2012 13:R14 doi:10.1186/gb-2012-13-2-r14 |
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